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Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 230-236 doi: 10.15302/J-FASE-2015071

Abstract: Large-scale production of cell culture-based classical swine fever virus (CSFV) vaccine is hampered byTFF concentrated the virus particles effectively with a retention rate of 98.5%, and 86.2% of viral particlesfrom 1.0×10 TCID ·mL to 3.0×10 TCID ·mL , but the combination of TFF and SEC produced more pure virusThe purification procedures in this study are reliable technically and feasible for purification of large

Keywords： classical swine fever virus     virus purification     tangential-flow filtration     size-exclusion chromatography    

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Bioinspired and biomimetic membranes for water purification and chemical separation: A review

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 6, doi: 10.1007/s11783-021-1412-8

Abstract:

•The history of biological and artificial water channels is reviewed.

Keywords： Aquaporins     Artificial water channels     Biomimetic membranes     Chemical separation and water purification    

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Advanced purification and comprehensive utilization of yellow phosphorous off gas

Ping NING,Xiangyu WANG

Frontiers of Environmental Science & Engineering 2015, Volume 9, Issue 2,   Pages 181-189 doi: 10.1007/s11783-014-0698-1

Abstract: Without appropriate purification and removal, this off gas has potential to cause severe pollution problemsIn this paper, the significance of purification and reutilization of yellow phosphorous off gas are exploredThe principles, processes, and main characteristics of the technologies for purification and reuse ofWe conclude that advanced purification and comprehensive reutilization can be an effective solution for

Keywords： yellow phosphorous off gas     purification     comprehensive utilization    

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Purification of

Y. LIU, M. PIETZSCH, J. ULRICH

Frontiers of Chemical Science and Engineering 2013, Volume 7, Issue 1,   Pages 37-42 doi: 10.1007/s11705-013-1303-z

Abstract: Here a case study of -asparaginase II out of a recombinant is presented. The target protein was obtained by simple cell disintegration and acetone precipitation. The -asparaginase II has been crystallized in three different forms in the following microbatch crystallization. The rod-shaped crystals (~400 μm edge length) were obtained at either 8°C or 22°C after 17 h by addition of PEG . The rectangular-shaped crystals were obtained after further recrystallization of the rod-shaped crystals. The rhombic-shaped crystals formed at 8°C after 12 days when cold ethanol was used instead of PEG . All crystallizations were performed in tris-acetate buffer (50 mmol·L , pH 5.1). By crystallization, the specific activity of -asparaginase II has increased 5-fold. The protein content and the purity of the crystals were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The more concentrated -asparaginase II out of an extract mixture and the presence of only less minor proteins after crystallization demonstrates that crystallization is an effective and mild method to purify the target protein. The single crystal X-ray diffraction pattern reveals that the crystals are proteins and the X-ray powder diffraction (XRPD) pattern shows clearly that the crystals forming in PEG and ethanol have different crystal structures.

Keywords： protein crystallization     L-asparaginase II     purification    

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A hybrid fuel cell for water purification and simultaneously electricity generation

Frontiers of Environmental Science & Engineering 2023, Volume 17, Issue 1, doi: 10.1007/s11783-023-1611-6

Abstract:

● A novel hybrid fuel cell (F-HFC) was fabricated.

Keywords： Flow-through field     Hybrid fuel cell     Polyoxometalates     Water purification     Electricity generation    

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Removal of virus aerosols by the combination of filtration and UV-C irradiation

Frontiers of Environmental Science & Engineering 2023, Volume 17, Issue 3, doi: 10.1007/s11783-023-1627-y

Abstract:

● The removal of virus aerosols by filtration and UV-C irradiation

Keywords： Filtration system     UV-C irradiation     Virus aerosol     Public health     COVID-19    

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Critical roles of chemokines and cytokines in antiviral innate immune responses during rabies virus infection

Ying HUANG, Clement Wesley GNANADURAI, Zhenfang FU

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 3,   Pages 260-267 doi: 10.15302/J-FASE-2016116

Abstract: pro-inflammatory chemokines and cytokines have a critical function in the innate immune responses against virusThe ability of a rabies virus (RABV) to induce the expression of chemokines and cytokines can lead to

Keywords： antiviral     blood brain barrier     chemokines and cytokines     innate immunity     rabies virus    

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Immunological and virological characteristics of human immunodeficiency virus type 1 superinfection:

Yang Gao, Wen Tian, Xiaoxu Han, Feng Gao

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 480-489 doi: 10.1007/s11684-017-0594-8

Abstract:

Superinfection is frequently detected among individuals infected by human immunodeficiency virus type

Keywords： human immunodeficiency virus type I     superinfection     incidence     immune response    

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Pressure swing adsorption/membrane hybrid processes for hydrogen purification with a high recovery

Baojun Li,Gaohong He,Xiaobin Jiang,Yan Dai,Xuehua Ruan

Frontiers of Chemical Science and Engineering 2016, Volume 10, Issue 2,   Pages 255-264 doi: 10.1007/s11705-016-1567-1

Abstract: The hybrid hydrogen purification processes were simulated using HYSYS and the processes were evaluatedcomparison, a PSA process and a membrane separation process were also used individually for hydrogen purification

Keywords： hydrogen purification     PSA     membrane separation     hybrid process    

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Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 158-163 doi: 10.1007/s11684-009-0042-5

Abstract: regulatory effect of basic fibroblast growth factor (bFGF) mediated by recombinant adeno-associated virusco-transfected into BHK-21 cells, then the recombinant AAV genome was replicated and packaged with the helper virusparticle titer of rAAV2-tet-off-bFGF successfully constructed was 1.8×10 vector genomes/mL, and the virus

Keywords： tetracycline regulatory system     adeno-associated virus     basic fibroblast growth factor     gene regulation    

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Advances in newly developing therapy for chronic hepatitis C virus infection

Paul J. Pockros

Frontiers of Medicine 2014, Volume 8, Issue 2,   Pages 166-174 doi: 10.1007/s11684-014-0334-2

Abstract:

Chronic hepatitis C virus (HCV) infection afflicts a reported 170 million people worldwide and is

Keywords： direct-acting antiviral (DAA)     nucleotide polymerase inhibitors     protease inhibitors    

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Universal influenza virus vaccines: what can we learn from the human immune response following exposure

Daniel Stadlbauer, Raffael Nachbagauer, Philip Meade, Florian Krammer

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 471-479 doi: 10.1007/s11684-017-0602-z

Abstract:

Several universal influenza virus vaccine candidates based on eliciting antibodies against the hemagglutininunderstanding of this phenomenon and the underlying mechanisms might help to develop future universal influenza virus

Keywords： universal influenza virus vaccine     hemagglutinin stalk     H7N9    

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Repurposing clinical drugs is a promising strategy to discover drugs against Zika virus infection

Weibao Song, Hongjuan Zhang, Yu Zhang, Rui Li, Yanxing Han, Yuan Lin, Jiandong Jiang

Frontiers of Medicine 2021, Volume 15, Issue 3,   Pages 404-415 doi: 10.1007/s11684-021-0834-9

Abstract: Zika virus (ZIKV) is an emerging pathogen associated with neurological complications, such as Guillain–Barré

Keywords： Zika virus     clinical drugs     ZIKV inhibitors     antivirals     repurposing    

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Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division

Claudia DOEL,Zhidong ZHANG,Lise MAZELET,Ryan WATERS,John BASHIRUDDIN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 250-257 doi: 10.15302/J-FASE-2014031

Abstract: Foot-and-mouth-disease virus (FMDV) replicates in epithelial cells.

Keywords： foot-and-mouth disease virus     cell cycle     replication    

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Chronic hepatitis B virus infection: epidemiology, prevention, and treatment in China

Rui Yu,Rong Fan,Jinlin Hou

Frontiers of Medicine 2014, Volume 8, Issue 2,   Pages 135-144 doi: 10.1007/s11684-014-0331-5

Abstract: The universal vaccination program since 1992 has changed the epidemiology of hepatitis B virus infectionThe most prevalent hepatitis B virus strains in China are genotypes B and C, whereas those in western

Keywords： chronic hepatitis B     epidemiology     prevention     treatment    

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Title Author Date Type Operation

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion

Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG

Journal Article

Bioinspired and biomimetic membranes for water purification and chemical separation: A review

Journal Article

Advanced purification and comprehensive utilization of yellow phosphorous off gas

Ping NING,Xiangyu WANG

Journal Article

Purification of

Y. LIU, M. PIETZSCH, J. ULRICH

Journal Article

A hybrid fuel cell for water purification and simultaneously electricity generation

Journal Article

Removal of virus aerosols by the combination of filtration and UV-C irradiation

Journal Article

Critical roles of chemokines and cytokines in antiviral innate immune responses during rabies virus infection

Ying HUANG, Clement Wesley GNANADURAI, Zhenfang FU

Journal Article

Immunological and virological characteristics of human immunodeficiency virus type 1 superinfection:

Yang Gao, Wen Tian, Xiaoxu Han, Feng Gao

Journal Article

Pressure swing adsorption/membrane hybrid processes for hydrogen purification with a high recovery

Baojun Li,Gaohong He,Xiaobin Jiang,Yan Dai,Xuehua Ruan

Journal Article

Regulation of exogenous bFGF gene mediated by recombinant adeno-associated virus

Ke SONG, Nianjing RAO, Meiling CHEN, Yingguang CAO

Journal Article

Advances in newly developing therapy for chronic hepatitis C virus infection

Paul J. Pockros

Journal Article

Universal influenza virus vaccines: what can we learn from the human immune response following exposure

Daniel Stadlbauer, Raffael Nachbagauer, Philip Meade, Florian Krammer

Journal Article

Repurposing clinical drugs is a promising strategy to discover drugs against Zika virus infection

Weibao Song, Hongjuan Zhang, Yu Zhang, Rui Li, Yanxing Han, Yuan Lin, Jiandong Jiang

Journal Article

Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division

Claudia DOEL,Zhidong ZHANG,Lise MAZELET,Ryan WATERS,John BASHIRUDDIN

Journal Article

Chronic hepatitis B virus infection: epidemiology, prevention, and treatment in China

Rui Yu,Rong Fan,Jinlin Hou

Journal Article